Genome-wide Identification and Expression Analysis of the PIN Gene Family during Abiotic Stress in Tomato (Solanum lycopersium)

摘要

It is widely believed that the PIN gene family is the most important auxin efflux carrier in plants since the separation of pin- formed mutant from Arabidopsis thaliana in 1991. Auxin transport and accumulation can be indirectly measured by the expression level of PIN genes. In recent years, members of the PIN gene family have been cloned in different species. Ten PIN genes (SlPINs) were identified in the genome data of tomato using the Arabidopsis PIN protein family for reference sequences. Phylogenetic analysis showed that 10 members were divided into “short” and “long” PINs. Chromosomal distribution map revealed that the SlPIN genes were unevenly distributed in eight chromosomes. We also studied the expression of 9 SlPIN genes under cold, drought and salinity by qRT-PCR, (SlPIN2 was not expressed in tomato leaves). The obtained levels of expression of the same genes were different under different stresses. Some genes were up regulated under some stresses but were down-regulated under other stresses. The relative expression of SlPIN6 clearly changed under cold and drought stress. The results here would provide the theoretical basis for the molecular cloning and resistance breeding of PIN genes in tomato.