Adsorption rule of five kinds of macroporous resin XDA-1,XDA-5,XAD-16,AB-8 and LH-20 which were from both domestic and abroad on apple polyphenols,desorption characters of XAD-16 were studied by dynamic adsorption to develop effective measure on both apple planting and separation and purification of plant polyphenols through macroporous resin.The results indicated that different types of macroporous resin had different maximum adsorption capacity (Max A) for apple polyphenol components and among that XDA-1 had the largest Max A for total phenols with a measured value of 38.90 mg/g and a model fitting value of 38.26 mg/g (R2 =0.968 4),LH-20 had the largest Max A for flavanols with a measured value of 37.75 mg/g and a model fitting value of 37.98 mg/g (R2 =0.980 6),XAD-16 had the largest Max A for proanthocyanidins with a measured value of 29.60 mg/g and a model fitting value of 30.05 mg/g (R2 =0.976 6).The equilibrium time for apple polyphenol components to achieve Max A varied with different types of macroporous resin:9 hours for total phenols to reach 98.43％ of Max A through XAD-16,while 10 hours for flavanols to reach 93.18％ of the Max A through AB-8 and proanthocyanidins to reach 94.86％ of Max A through XDA-5.Desorption efficiency of apple polyphenol components also significantly varied according to different concentrations of ethanol when XAD-16 reached adsorption equilibrium and the ratio for 50％ ethanol to desorb total phenols was 95.05％-105.29％,while for 70％ ethanol to desorb both total phenols and proanthocyanidins it went over 90％.In was demonstrated that XAD-16 macroporous resin with 50％ ethanol as the desorption liquid was s good choice to separate and purify apple polyphenols.%为有效开发苹果产业的可利用资源,进行植物多酚大孔树脂分离、纯化的工业化生产,采用动态吸附法,比较研究了XDA-1,XDA-5,XAD-16,AB-8,LH-20国内外5种不同大孔树脂对苹果多酚的吸附规律以及XAD-16的解吸特性.结果表明,大孔树脂的类型不同,对苹果多酚各组分的最大吸附量不同,其中,XDA-1对总酚的最大吸附量最大,试验测定值与模型拟合值分别为38.90,38.26 mg/g(R2=0.968 4);LH-20对黄烷醇类物质的最大吸附量最大,试验测定值与模型拟合值分别为37.75,37.98 mg/g(R2=0.9806);XAD-16对原花色素的吸附量最大,试验测定值与模型拟合值分别为29.60,30.05 mg/g(R2=0.976 6);苹果多酚各组分达到最大吸附量所需要的平衡时间因大孔树脂类型的不同而异,其中,XAD-16在9h达到总酚最大吸附量的98.43％,AB-8在10 h达到黄烷醇最大吸附量的93.18％,XDA-5在10 h达到原花色素最大吸附量的94.86％;XAD-16达到吸附平衡后,乙醇浓度不同对苹果多酚各组分的解吸效率存在显著差异,使用浓度为50％乙醇作为解吸液,1h对总酚的解吸率为95.05％-105.29％,使用浓度为70％的乙醇作为解吸液,可使总酚和原花色素的解吸率达90％以上.建议选择使用XAD-16大孔树脂,并配合使用50％乙醇作为解吸液进行苹果多酚的分离纯化.
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