转录因子FoxO3a磷酸化对血管平滑肌细胞增殖能力的影响

摘要

Objective To observe the effects of transcription factor FoxO3a phosphorylation on the proliferation of vascular smooth muscle cells after common carotid artery balloon injury.Methods Thirty male SD rats were randomly divided into control group ( group C, n = 6) and balloon injury group ( group S, n = 24), and the latter was subdivided into group S1 (6 h after surgery, n =6), group S2 ( 12 h after surgery, n =6), group S3 (24 h after surgery, n =6) and group S4 (72 h after surgery, n = 6).Left common carotid artery balloon injury was performed in rats of group S, the injured arteries were harvested to culture the vascular smooth muscle cells, the expression of FoxO3a mRNA was detected by RT-PCR, the expression of FoxO3a protein was detected by western blotting, and the cell proliferation was determined by MTT.Results Compared with group C, group S2, group S3 and group S4, the expression of phosphorylated FoxO3a protein in vascular smooth muscle cells in group S1 increased significantly after vascular injury (P ＜ 0.01).Compared with group C, the cell proliferation in group S1 increased significantly 2 d, 3 d, 4 d and 5 d after culture (P ＜ 0.01).Conclusion FoxO3a is quickly phosphorylated after vascular injury.The duration of phosphorylation may last for a short while, and may be associated with the significant increase of proliferation of vascular smooth muscle cells.It is suggested that the phosphorylation of FoxO3a may play an important role in the repair of vascular injury%目的 观察转录因子FoxO3a磷酸化对颈总动脉球囊损伤后血管平滑肌细胞增殖能力的影响.方法 30只雄性SD大鼠分成对照组(C组)和球囊损伤组(S组),其中球囊损伤组又分为损伤后6、12、24、72 h组(S1、S2、S3、S4组,n=6).S组大鼠均行左侧颈总动脉球囊损伤术,取损伤处血管组织培养平滑肌细胞,采用RT-PCR技术检测FoxO3a mRNA表达,Western blotting法检测FoxPO3a蛋白表达,MTT法检测细胞的增殖能力.结果 颈动脉损伤后S1组血管平滑肌细胞的FoxP3a磷酸化蛋白表达明显增强,与C组和其他S组比较差异均有统计学意义(P<0.01);培养后第2、3、4、5天,S1组血管平滑肌细胞的增殖能力显著高于C组,差异有统计学意义(P<0.01).结论 血管损伤后,FoxO3a快速发生磷酸化,持续时间短,可能与伴随的血管平滑肌细胞增殖能力显著升高相关联,提示FoxO3a的磷酸化可能在血管损伤后的修复中发挥重要作用.