响应曲面法优化酸奶中嗜热链球菌 H1的生长条件

摘要

采用单因素试验和响应曲面法对酸奶中嗜热链球菌 H1的最适生长条件进行了研究和优化．根据回归分析得到的最适培养条件为：温度41．02℃，pH5．74，接种量0．31mL ·50mL -1，盐浓度0．23％，此时菌株的光密度OD600为1．176．经实验验证，在该条件下菌株光密度OD600为1．162，其相对误差小于2％．%The growth conditions of S .thermophilus H1 were studied by single factor experiment and response surface methodology . The results showed that the optimal parameters for the growth conditions as follows :the temperature was 41 .02℃ ,the pH was value 5 .74 ,the inoculum size was 0 .31mL · 50mL -1 ,the salt concentration was 0 .23% ,and the OD600 of strain HI was 1 . 176 .Under the optimal conditions the OD600 of strain HI was 1 .162 ,The relative error was bel-lo w 2% .