植物叶片酚酸类物质微量提取分离与GC-MS分析

摘要

建立了一种微量提取植物新鲜叶片中肉桂酸、香豆酸、阿魏酸和芥子酸的方法。首先由甲醇：水：甲酸提取缓冲液过夜抽提新鲜样品,以4-甲氧基苯甲酸作为内标,利用酚酸类物质在不同pH值溶液中的溶解性不同,分别用乙酸乙酯和5%NaHCO3溶液初步纯化提取物;由自制的微量C18柱纯化提取液,并确定45%甲醇/0.1M醋酸的柱洗液回收效率最高。建立了气相—质谱联用仪同时分析4种酚酸的程序方法,确定了4种酚酸的硅烷化温度和时间,并通过提取杨树、银杏、杜仲、华山松4种树木新鲜叶片的酚酸物质验证此方法的可行性。%Trans-p-coumaric,trans-cinnamic,trans-ferulic and trans-sinapic were identified by means of GC-MS method isolated by means of carbon 18 column from leaves of Populus tomentosa,Ginkol biloba,Eucommia ulmoides Oliv.and Pinus armandii Franch.The compounds were distilled by methyl alcohol：H2O：formic acid（15：4：1）from finely ground leaves and the aqueous acidic solution was extracted with ethyl acetate and 5% NaHCO3 alternately.After filtration,the acids dissolved in 45%methyl alcohol/0.1 M acetic acid washed cross the C18 column were collected and dryness in nitrogen.