实验室因素对胚胎发育和后代的影响

摘要

胚胎发育是非常复杂但变化极为协调的生理过程.体外受精及胚胎培养的过程中,环境中多种理化因素都可能影响胚胎发育过程甚至导致后代发育异常.本文就实验室中可能对胚胎发育造成影响的各项因素进行综述,主要包括空气质量、温度、pH值、培养箱类型、氧气浓度、培养液类型、胚胎培养密度等方面,以期完善胚胎培养体系,最大程度上减少实验室因素对胚胎发育的不良影响,改善人类辅助生殖技术治疗的结局.%Embryo development is a very complex and highly coordinated physiological process.During the procedure,a variety of factors in the external environment may cause abnormal development of embryos and offspring.With the continuous development and improvement of assisted reproductive technology (ART),the impact of various factors on the development of embryos and offspring has also been paid more attention by scholars.This review mainly focuses on the laboratory factors that affect the development of embryos and offspring,including air quality,temperature,pH value,incubator,culture density,culture medium,oxygen concentration and so on,in order to minimize the adverse effects of laboratory factors on human ART and improve the clinical outcome.