镇肝熄风汤对帕金森模型小鼠脑黑质 Bcl-2家族mRNA 表达的影响

摘要

目的：观察镇肝熄风汤对1-甲基-4-苯基-1，2，3，6-四氢吡啶（1-methy-4-phenyl-1，2，3，6-tetrahy-dropyridine ，MPTP）诱导的帕金森病（ PD）模型小鼠脑黑质Bcl-2家族蛋白表达的影响。方法采用连续灌胃给予C57BL/6小鼠不同剂量镇肝熄风汤（12．5、25和50 g/kg）14天，于第11天开始，给药前1 h皮下注射MPTP（30 mg/kg），连续4天，造模后运用实时定量PCR法检测Bax、Bad、Bcl-2和Bcl-xl mRNA的表达。结果 PCR结果显示，镇肝熄风汤下调了Bax mRNA表达（P＜0．05），同时上调了Bcl-2和Bcl-xl mRNA表达（P＜0．05），对Bad mRNA表达无影响。结论镇肝熄风汤具有抑制PD模型小鼠脑黑质Bax mRNA表达，促进Bcl-2和Bcl-xL mRNA表达的作用。%Objective To investigate the effects of Zhenganxifeng decoction on expression of Bcl -2 family mRNA in the substantia nigra of MPTP-induced C57BL/6 mice.Methods C57BL/6 mice were given MPTP (30 mg/kg) to prepare Parkinson disease mice model .Different doses of Zhenganxifeng decoction (12.5, 25, and 50 g/kg) were given 14 d prior to MPTP in the pretreatment groups .Real-time PCR was used to analyze the expression of Bax , Bad, Bcl-2 and Bcl-xl mRNA.Results Zhenganxifeng decoction could down-regulate the expression of Bax mRNA , and up-regulate the expression of Bcl-2 and Bcl-xL mRNA.Conclusions The results suggest that Zhenganxifeng decoction could suppress the expression of Bax mRNA and Promote the expression of Bcl-2 and Bcl-xl mRNA in substantia nigra of Parkinson's disease mice induced by MPTP .