IL－10对重症急性胰腺炎大鼠血清 IL－2、IL－8及胰腺组织的影响

摘要

目的：探讨大鼠血清中IL－2、IL－8及IL－10等细胞因子在重症急性胰腺炎（ SAP）中的作用，为临床治疗SAP提供相关理论依据。方法 Wistar大鼠120只，分为三组。 SAP组：6％的L－arginine诱导大鼠SAP；IL－10组：造模后腹腔内注射IL－10；C组：各时间点给予生理盐水作为正常对照。分批处死大鼠，ELISA检测血清IL－2、IL－8、IL－10的水平，并对胰腺组织进行组织病理学评分。结果 IL－10组血清IL－2、IL－8水平及胰腺病理评分在多个时间点显著高于C组并且低于SAP组（P＜0．05），而IL－10显著高于C组和SAP组（P＜0．05）。结论（1）血清IL－2、IL－8是促炎性细胞因子，与SAP的病变程度正相关；IL－10是抗炎性细胞因子，与SAP的病变程度负相关。（2）IL－10可作为潜代药物应用于急性胰腺炎的治疗。%Objective To investigate the effects of interleukin-2, interleukin-8 and interleukin-10 of serum in severe acute pancreatitis (SAP), to provide a new approach for clinical treatment and theory basis for SAP.Methods 120 wistar rats were randomly divided into three groups .Group SAP received 6%L-arginine to induce SAP model;Group IL-10 also received L-arginine and injection IL-10 after the last injection of L-arginine. Group C only received physiological saline .Then killed the rats, serum IL-2, IL-8 and IL-10 were assayed by ELISA, and pancreatic tissues were fixed in formalin .Histopathological score was recorded in three groups . Results The level of IL-2, IL-8 and histopathology scores were higher than group C and lower than group SAP several times (P<0.05), however, IL-10 was higher than group C and group SAP (P<0.05).Conclusions IL-2 and IL-8 are able to promote the severity of SAP , however , IL-10 have the opposite effect .Maybe IL-10 can be a potential drug for the clinical treatment of acute pancreatitis .