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崂山奶山羊 BM PR-IB 基因的多态性分析

     

摘要

One hundred Laoshan milk goats were randomly selected for investigation of polymorphism of BMPR-IB genes by PCR-SSCP .The results suggested the mutation of G→C at the site of 820 bp of the exon of BMPR-IB gene ,and the frequency of allele A and allele B was 0.855 and 0.145 ,respectively ,loci heterozygosity was 0.248 ,the effective number of alleles was 1.330 ,and the polymorphism information content PIC was 0.217 .The findings could provide a reference for MAS programs for high reproduction performance of Laoshan Milk Goat breeds .%为分子标记辅助选择崂山奶山羊高繁殖力提供依据,对100只崂山奶山羊耳组织采样,利用PCR-SSCP技术对其BM PR-IB基因外显子进行多态性分析。结果表明,崂山奶山羊BM PR -IB基因外显子的820bp位点出现了G→C的突变,A与B等位基因的频率分别为0.855、0.145,此突变位点杂合度为0.248,有效等位基因数为1.330,多态信息含量PIC为0.217,为低度多态。

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