野黄芩苷对人舌鳞癌Tca8113细胞周期及Fas蛋白表达的影响

摘要

Objective: To investigate the effects of scutellarin on the Fas expression and cell cycle distribution of human tongue squamous cell carcinoma Tca-8113 cells. Methods: Tca-8113 cells were cultured in vitro and treated by scutellarin at various concentrations. Flow cytometry was used to examine the cell cycle distribution. Western blot was used to detect the Fas expression of the cells. Results: Scutellarin at 80, 120 and 160 μg/ml increased the percentage of G1-phase cells (P <0. 05) and at 160 μg/ml decreased the percentage of S-phase cells(P <0.05). Scutellarin at 80, 120 and 160 μg/ml increased the Fas expressions(P<0.05). Conclusion: Scutellarin may inhibit the proliferation and enhance the Fas expressions of human tongue squamous cell carcinoma Tca-8113 cells.%目的:观察野黄芩苷对人舌鳞癌Tea8113细胞周期及Fas蛋白表达的影响.方法:用浓度为80、120和160 μg/ml的野黄芩苷处理体外培养的人舌鳞癌Tea8113细胞,用流式细胞技术观察细胞周期的变化；用蛋白印迹法定量检测Fas蛋白表达.结果:与对照组相比,加入不同浓度野黄芩苷后DNA合成前期(G1)细胞所占百分比(G1％)升高(P＜0.05),160 μg/ml野黄芩苷处理后DNA合成期(S)细胞所占百分比(S％)降低(P＜0.05).蛋白印迹法结果显示,随着药物浓度的增加,Fas蛋白表达量有增高趋势,与对照组比较,差异有统计学意义(P＜0.05).结论:野黄芩苷可能通过抑制肿瘤细胞增殖,提高人舌鳞癌Tca8113细胞Fas蛋白的表达而达到抗肿瘤的作用.