纯钛经苯扎氯铵浸泡后的抗菌性能的研究

摘要

Objective:To study the antibacterial properties of pure titanium treated with benzalkonium chloride solution.Meth-ods:10 mm ×10 mm ×1 mm titanium specimens were processed by the benzalkonium chloride solution at 1%,0.5% and 0.1%respectively followed by treatment in the cultured bacterial suspension,and then the antibacterial properties of the titanium plates were examined.Additionally,the thermal cycling test was carried out for the 1% benzalkonium chloride-treated titanium plates, and subsequently put the plates into cultured bacterial suspension,the duration of antibacterial properties was observed.Results:0.5% and 1% benzalkonium chloride solution-treated titanium plates significantly inhibited the growth of candida albicans(P <0. 05),1% solution was more effective than 0.5% solution.After 1 000 and 2 500 thermal cycling,the pure titanium still retained the antibacterial ability,but the plates treated by 5 000 cycling showed no antibacterial effect.Conclusion:A certain concentration of benzalkonium chloride can make the pure titanium obtain antibacterial properties.The treated plates may maintain the antibacte-rial properties for a minimum of 3 months.%目的：通过用苯扎氯铵溶液浸泡处理钛片，了解该方法能否使纯钛具有抗菌性及抗菌性持续的时间。方法：制作10 mm ×10 mm ×1 mm 的钛片试件，用不同浓度的苯扎氯铵溶液浸泡处理，菌悬液浸泡培养，检测有无抗菌性。用1％的苯扎氯铵溶液浸泡处理试件，进行冷热循环实验后再进行细菌培养，检测抗菌性的持续时间。结果：不同浓度的苯扎氯铵溶液处理纯钛后对白色念珠菌生长的抑制效果不同，0．5％和1％的苯扎氯铵溶液浸泡纯钛可使其产生抗菌性（均有显著差异，P ＜0．05），1％的苯扎氯铵溶液效果更佳。冷热循环1000次和2500次后纯钛仍具有抗菌性，5000次时与未进行抗菌处理效果相同。结论：一定浓度的苯扎氯铵溶液浸泡纯钛后，可使纯钛具有抗菌性，其抗菌性至少可以持续3个月左右。