乙型肝炎病毒PreS1抗原、丙氨酸氨基转移酶和HBV-DNA相关性分析

摘要

Objective To investigate the correlations between HBV preSlAg, ALT and quantitative expression of HBV-DNA, respectively. Methods Serum samples from 4 439 patients in hospital or clinic were collected, then the HBV-DNA was detected by fluorescence quantitative PCR method, PreSlAg was measured by ELISA, and ALT was detected by rate method. After that, 186 patients with HBV-DNA positive expression were included as the quantitative detection objects, then the relative titer value of PreSlAg and common logarithmic value of HBV-DNA were calculated. Results In 4 439 specimens, the positive rates of PreSlAg and HBV-DNA were 21.27% and 21.76%, the abnormal rate of ALT was 21.36% (P > 0.05). Among the 186 patients with HBV-DNA positively expressed, there was no correlations between the relative titer value of PreSlAg (17.53 ± 14.17), ALT (64.96 ± 175.89) and quantitative logarithmic value of HBV-DNA (6.27 ± 1.14). Conclusion PreSlAg may be a good new laboratory parameter for detecting HBV virus infection, but cannot account for replication. United detection of PreSlAg and ALT was important for clinical diagnosing and treating patients with HBV infection.%目的:探讨PreS1抗原、丙氨酸氨基转移酶(ALT)分别与HBV-DNA定量表达的相互关系.方法:选取住院和门诊受检者4 439例血清标本,分别用荧光定量PCR法检测HBV-DNA,ELISA法检测PreS1抗原,速率法检测ALT;从其中选取HBV-DNA阳性186例作为定量检测对象,并计算PreS1抗原相对滴度值和HBV-DNA常用对数值.结果:4439例标本中PreS1抗原阳性率为21.27%,ALT异常率为21.36%,与HBV-DNA的阳性率(21.76%)都无显著差异(P>0.05);186例HBV-DNA阳性标本中PreS1相对滴度(17.53±14.17)、ALT(64.96±175.89)与HBV-DNA定量对数值(6.27±1.14)均不相关.结论:检测PreS1抗原可以作为HBV病毒感染和复制的较好的新指标,但不能说明复制量情况;联合检测ALT对HBV感染患者的诊断、治疗有重要的价值.