Peroxiredoxin Ⅳ mRNA在小鼠卵泡发育和卵巢衰老过程中的表达

摘要

Objective To explore the expression of peroxiredoxin Ⅳ mRNA in the follicle development and ovarian aging of mice.Method C57BL/6j mice in different stages of age and estrous cycle were pretreated with gonadotropin.Then real-time polymerase chain reaction was used to detect the expression of PRDX Ⅳ mRNA.Results The expressions of PRDX Ⅳ mRNA in the ovaries of mice in 3 weeks old,6 weeks old,and 8 months old were 0.76,0.42 and 0.12 of those in 3 days old (P ＜ 0.05) ; And were not significantly different among matured mice in different estrous cycle.In immature mice pretreated with gonadotropin,the expression of PRDX at 48 h after PMSG injection was 1.68 of that after normal saline injection (CON group) (P ＜ 0.05) ; and those at 4,8,12,48 h after HCG injection were 0.81,0.56,0.78,0.66 of those in CON group (P ＜ 0.05).Conclusion PRDX Ⅳ may probably regulate ovarian aging through regulating follicle development and ovarian oxidative stress.%目的:探讨Peroxiredoxin (PRDX)ⅣmRNA在小鼠卵泡发育和卵巢衰老过程中表达.方法:选用C57BL/6j小鼠不同周龄、动情周期以及促性腺激素预处理卵巢,通过实时定量聚合酶链反应检测PRDXⅣmRNA表达.结果:PRDXⅣmRNA在3周龄、6周龄、8月龄卵巢中表达分别为新生3d小鼠的0.76、0.42、0.12 (P＜ 0.05)；在性成熟小鼠各动情周期卵巢中表达无差异；在未成熟小鼠促性腺激素预处理卵巢中,PMSG注射48 h表达为生理盐水注射组(CON组)的1.63倍(P＜0.05),于HCG注射后4、8、12、48 h分别为CON组的0.81、0.56、0.78、0.66(P＜ 0.05).结论:PRDXⅣ可能通过调节卵泡发育与卵巢氧化应激压力而调控卵巢衰老过程.