Objective To investigate radiosensitization effect of valproic acid on human glioma carcinoma cell line SHG-44 and its mechanism. Methods SHG-44 cells were treated with 0.5, 1.0, 2.0, 4.0 and 8.0 mmol/L sodium valproate, respectively. The radio sensitization of valproic acid on SHG-44 cells was measured by colony formation assay. The proliferation inhibition rate of valproic acid on was investigated by MIT assay in different time and dose. The apoptosis and cycle of SHG-44 cells in different groups were analyzed by flow cytometry (FCM). Results After treated with valproic acid, the cell proliferation was inhibited significantly in a time-and dose-dependent manner. The valproic acid have radiosensitization on human glioma carcinoma cell line SHG-44, SER = 1.32. Compared with the control group, cell apoptosis and the proportion of G0/G1 phase were increased significantly in the combination group, and the proportion of S phase was reduced that assayed by FCM. Conclusion Valproic acid can increase the radiosensitization of SHG-44. Its role may be to induced cell apoptosis, change cell cycle phase distribution and other mechanisms.%目的:研究丙戊酸钠对人脑胶质瘤细胞系SHG-44的放疗增敏作用及其可能机制.方法:应用0.5、1.0、2.0、4.0、8.0 mmol/L丙戊酸钠干预体外培养的SHG-44细胞,MTT法检测不同时间及不同剂量丙戊酸钠对SHG-44细胞的抑制率、以克隆形成率实验检测丙戊酸钠对SHG-44细胞的放射增敏比、流式细胞术检测细胞凋亡及周期差异.结果:丙戊酸钠明显抑制细胞增殖,并具有时间及剂量依赖性,并且对胶质瘤细胞株SHG-44具有放疗增敏作用,放射增敏比为1.32;流式细胞术分析见细胞凋亡,联合组较对照组明显增加,细胞周期中联合组G0/G1期细胞比例升高,S期细胞比例降低,联合组与各单独作用组相比差异显著.结论:丙戊酸钠能增强SHG-44细胞放疗敏感性,其作用可能是通过诱导细胞凋亡、改变细胞周期时相分布等机制来实现的.
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