目的：探讨芦荟多糖对实验性结肠炎大鼠肠黏膜的保护作用。方法：成功建立2，4，6－三硝基苯磺酸（TNBS）实验性大鼠结肠炎模型，给予芦荟多糖治疗，并与柳氮磺吡啶肠溶片（SASP）做对照。结果：模型组大鼠DAI评分和组织病理学评分均高于正常组（P ＜0．01），而各给药组经治疗后各项指标均降低（P ＜0．01）。模型组IL-12和IFN-γ mRNA和蛋白表达水平高于正常组（P ＜0．01），而各给药组IL-12和IFN-γ表达水平低于模型组（P ＜0．01）。模型组IL-4和IL-13 mRNA和蛋白表达水平低于正常组（P ＜0．01），而各给药组IL-4和IL-13 mRNA 表达水平高于模型组（P ＜0．01）。结论：芦荟多糖对实验性结肠炎大鼠结肠黏膜有良好的保护作用，作用机制可能通过调节肠黏膜局部免疫平衡来发挥作用的。%Objective To investigate the protective effect of aloe polysaccharide on experimental colitis mucosal. Methods The TNBS-induced experimental rat colitis model was successfully established and were treated by aloe polysaccharide, and enteric-coated tablets with sulfasalazine (SASP). Results DAI score, gross morphology score , histopathologic score and MPO in the model group were significantly higher than that in the normal group (P < 0.01), while those indicators were significantly lower after treatment (P < 0.01), IL-12 and IFN-γ mRNA and protein expressions in the model group were significantly higher than that in the normal group (P < 0.01), and IL-12 and IFN-γ expression levels in treatment group were significantly lower than that in the model group (P < 0.01), IL-4 and IL-13 mRNA and protein expression levels in the model group were significantly lower than that in the normal group (P < 0.01), and IL-4 and IL-13 expression in treatment group was significantly higher than that in the model group (P < 0.01). Conclusion Aloe polysaccharide on experimental colitis in rats has a good protective effect , of which mechanism may be reduced by IFN-γ and IL-12 levels, increased IL-4, IL-13 levels, and correct the imbalance of Thl/Th2.
展开▼
