乙型肝炎病毒相关性肝癌组织维生素D受体表达情况分析

摘要

Objective To explore the expression of vitamin D receptor(VDR)in cancerous tissues of patients with hepatitis B virus(HBV)-related hepatocellular carcinoma(HCC). Methods The relative levels of VDR mRNA in liver tissues was detected by real -time PCR. VDR expression were determined by immunohistochemical method,and the relative expression of VDR protein was detected by Western blot. Results Immunohistochemistry showed that cancerous tissues,para-cancerous tissues and normal liver tissues expressed VDR equally. VDR was mainly expressed in the cytoplasm,and there was almost no expression in the nucleus. The RT-PCR detection showed that VDR mRNA in cancerous tissues was(2.77±0.30),significantly higher than that in the para- cancerous tissues(1.62±0.21)or normal liver tissues [(1.57±0.19),P<0.01];Western blot showed that the expression of VDR protein in cancerous tissues was(1.15±0.57),significantly higher than that in the para- cancerous tissues(1.02±0.25)or in normal liver tissues [(0.37±0.11),P<0.01]. Conclusion VDR is closely related to the development of hepatocellular carcinoma.%目的 探讨乙型肝炎病毒相关性肝细胞癌(HCC)组织维生素D受体(VDR)表达变化及其与癌旁肝硬化组织和正常组织的异同.方法 取10例乙型肝炎病毒相关性HCC组织及其癌旁肝组织和4例正常肝组织,采用实时荧光定量PCR法检测组织VDR mRNA水平,采用免疫组织化学法检测组织VDR表达,采用Western blot法检测组织VDR蛋白表达.结果 免疫组织化学法检测发现HCC组织、癌旁组织和正常肝组织均有VDR表达,主要在细胞质表达,而细胞核基本无表达;癌组织VDR mRNA为(2.77±0.30),显著高于癌旁肝硬化组织的(1.62±0.21)或正常肝组织的[(1.57±0.19),P<0.01];Western blot分析发现,HCC组织VDR蛋白相对表达量为(1.15±0.57),显著高于癌旁肝硬化组织的(1.02±0.25)或正常肝脏组织的[(0.37±0.11),P<0.01].结论 VDR可能参与了HCC的发生发展过程.