肝癌组织中VEGF-C、VEGF-D和受体VEGFR-3的表达及其相关性分析

摘要

Objective To explore the expression of vascular endothelial growth factor C (VEGF-C), vascular endothelial growth factor D (VEGF-D) and vascular endothelial growth factor receptor 3 (VEGFR-3) in liver cancer tissue and their role of the progression of liver cancer. Methods Liver tissues of 40 patients with hepatocellular carcinoma treated in Nanxishan Hospital of Guangxi Zhuang Autonomous Region ("our hospital" for short) from August 2012 to August 2014 and their para-carcinoma tissues were collected, normal liver tissues of 10 patients with hemangioma in our hos-pital at the same period were collected. The expression of VEGF-C, VEGF-D and VEGFR-3 of liver tissues were test-ed by immunohistochemical method, the relationship between VEGF-C, VEGF-D and VEGFR-3 in liver tissues were analyzed. Results VEGF-C and VEGF-D was mainly expressed in the cytoplasm of liver cancer cells, and their expres-sion in liver cancer tissues was significantly higher than para-carcinoma tissues and normal liver tissues (P< 0.05). VEGFR-3 was mainly expressed in the cancer cells of base parts of the liver tissues and VEGFR-3 positive rate of the liver cancer tissues was obviously higher than those of the normal liver tissues and para-carcinoma tissues (P< 0.01). The expression of VEGFR-3 was positively correlated with VEGF-C and VEGF-D (r = 0.546, 0.369; all P< 0.01). Conclusion VEGF-C, VEGF-D and VEGFR-3 are high expression in liver cancer tissues, and it may be associated with the development of the liver cancer. The joint detection can be used as an important index of judging liver cancer occurrence and metastasis.%目的：探讨肝癌组织中血管内皮生长因子-C（VEGF-C）、血管内皮生长因子-D（VEGF-D）和血管内皮生长因子受体-3（VEGFR-3）的表达及其在肝癌进展中的作用。方法收集2012年8月~2014年8月在广西壮族自治区南溪山医院（以下简称“我院”）住院行手术治疗的40例肝癌患者的癌组织及其无癌癌旁肝脏组织，同时选择我院同期收治的10例血管瘤患者的肝脏组织。采用免疫组织化学方法检测肝脏组织中VEGF-C、VEGF-D和受体VEGFR-3的表达，分析VEGF-C、VEGF-D和受体VEGFR-3表达在肝癌组织中的相关性。结果 VEGF-C和VEGF-D主要表达于肝癌细胞浆内，它们在肝癌组织中的表达明显高于癌旁组织及正常肝脏组织（P<0.05）；VEGFR-3主要表达于肝脏组织基底层部位的癌细胞，VEGFR-3在肝癌组织中的阳性表达率明显高于癌旁组织及正常肝脏组织（P<0.01），并且VEGFR-3的表达与VEGF-C、VEGF-D的表达呈正相关（r=0.546、0.369，均P<0.01）。结论 VEGF-C、VEGF-D和受体VEGFR-3在肝癌组织中表达明显增高，其可能与肝癌的发生发展有关，联合检测可作为判断肝癌发生和转移的重要指标。