蒲地灌肠液中总多糖含量测定与方法学验证

摘要

目的 建立蒲地灌肠液中总多糖的含量测定方法.方法 采用苯酚-硫酸法显色,紫外分光光度法在488.8 nm波长处测定总多糖的含量.结果 总多糖在0~22.635 mg/L范围内呈良好线性关系;其回归方程为Y=0.062 06 X-0.003 34(r=0.999 8),回收率为98.36%,RSD为2.34%(n=6).结论 该方法灵敏、快速、准确、可靠,可用于蒲地灌肠液中总多糖含量的测定.%Objective To establish the assay method for the total polysaccharide in Pudi Enema.Methods Phenol-sulfuric acid method was used for chromogenic reaction.The content of total polysaccharide was measured by UV spectrophotometry at 488.8 nm.Results The total polysaccharides calibration curve was at the range of 0~22.635 mg/L, with regression function being Y=0.062 06 X-0.003 34(r=0.999 8).The recovery of calycosin was 98.36%(RSD=2.34%).Conclusion This method is sensitive,rapid,accurate and reliable.It can be used to assay the content of total polysaccharide in Pudi Enema.