拟南芥ERF转录因子基因应答非生物胁迫表达模式

摘要

以122条拟南芥（ Arabidopsis thaliana） ERF转录因子家族基因为研究对象，用RT－qPCR检测其应答盐胁迫情况，分析15个盐胁迫敏感基因在盐、干旱和ABA胁迫条件下的表达模式。在盐胁迫条件下，有36个基因上调表达，42个基因下调表达，44个基因无明显变化。其中，15个盐胁迫敏感基因在不同胁迫条件下表现出不同的表达模式。在盐胁迫条件下，15个ERF基因的表达水平随胁迫时间的延长均表现出明显升高，在36和72 h两个时间点达到极显著水平。在ABA胁迫条件下，15个ERF基因中大多数表达水平提高。在干旱胁迫下，15个ERF基因表达水平均随胁迫时间延长有所提高，在48 h 达到最高。 AT1G06160、A T1G21910、A T2G35700、AT4G17490、AT4G39780和AT5G61890等6个基因在非生物胁迫条件下表达模式相似，表明其可能参与相似的基因调控途径。其他9个ERF基因在非生物胁迫下表现出不同的表达模式，表明其可能参与不同基因调控途径。%The expression of 122 Arabidopsis ERF transcription factor genes under salt stress were detected by RT-qPCR, and the expression patterns of 15 salt-sensitive genes under salt, drought and ABA stress were analyzed.Under salt stress, 36 genes were up-regulated, 42 genes were down-regulated, and 44 genes had no significant change.Among them, 15 salt-sensitive genes showed different expression patterns under different abiotic stress.Under salt stress, the expression level of 15 ERF genes with the increasing of stress time was significantly increased, reaching a significant level at 36 h and 72 h. Under the ABA stress, expression level in most of 15 ERF gene was increased.Under drought stress, expression levels of 15 ERF gene were increased with the stress time prolonged, reaching the highest point at 48 h. AT1G06160, AT1G21910, AT2G35700, AT4G17490, AT4G39780 and AT 5G61890 genes were with the similar expression patterns under abiotic stres-ses, indicating that it may be involved in similar gene regulation pathways.The other 9 ERF genes under abiotic stress showed different expression patterns, indicating that it may be involved in different pathways of gene regulation.