交联聚乙二醇二丙烯酸酯固定化漆酶的性质

摘要

为探究交联聚乙二醇二丙烯酸酯为载体的固定化漆酶的优势,采用单因素分析法,在不同条件下对固定化漆酶和游离漆酶的酶学性质进行了研究.结果表明:与游离漆酶相比,固定化漆酶与底物2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS)的亲和力下降,但稳定性明显提高,最适pH值也发生了变化.固定化漆酶的最适温度为60℃,比游离漆酶升高了5℃;固定化操作pH为3.5时,固定化漆酶的酶活力最高.固定化漆酶对2,4-二氯苯酚降解时间为24h时,降解率达到了85%,酶活力保持80%以上,漆酶的使用效率和稳定性明显提高.%The experiment was conducted to study the advantage of laccase immobilization with the carrier being cross-linked polyglycol diacrylate.The control variate method was used to investigate the activity between laccase immobilization and free laccase from different conditions.Comparing with free laccase, appetency between immobilized laccase and 2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate) (ABTS) descends, but the stability was improved and the optimum pH changed.The pH effect in the immobilization process was conducted simultaneously.The proper temperature of immobilized laccase was 60℃ and 5℃ higher than that of free laccase.The activity of immobilized laccase got the highest level when pH was 3.5.At last, the immobilized laccase was used in the biocatalysis of 2,4-dichloro-phenol.Results indicate that the degradation ratio achieved 85% in 24h, and the kept activity was more than 80% with better use efficiency and stability of laccase.