膜荚黄芪内生真菌的分离与鉴定

摘要

根据膜荚黄芪（Astragalus membranaceus （Fisch．） Bunge）根叶组织在4种培养基上的生长状况，制成水载片，用光学显微镜定期观察产孢的内生真菌菌丝结构、菌落形态特征等指标鉴定到属。结果表明：从膜荚黄芪根、叶各培养基上共分离65株内生真菌。经显微形态观察鉴定为半知菌门镰孢属、曲霉属、青霉属，其中镰孢属为优势菌群，且大都无孢子产生。%The experiment was conducted to make a water slide according to growth status, the root and leaf tissue of Astragalus membranaceus Bge.culture in four kind medium and classify the genus by observing to mycelial structure and colonial mor-phology during endophytic fungi spore production with optical microscopy.65 strains of endophytic fungi were isolated from astragaus root and leaf tissue in different culture medium.By the microscopic observation, the fungi are Fusarium, Penicil-lium and Aspergillus from Deuteromycota.Fusarium is dominant bacterium group without spore.