MiRNA-155在肝细胞癌组织中的表达及其与临床病理特征和预后的关系

摘要

目的：观察微小 RNA-155（miRNA-155）在肝细胞癌（hepatocellular cancer，HCC）组织中的表达及其与临床病理特征和预后的关系。方法：应用实时定量反转录 PCR检测77例 HCC 患者癌组织和癌旁组织中 miRNA-155表达情况，并分析不同病理特征 HCC 患者间 miRNA-155表达差异。Kaplan-Meier生存曲线分析 miRNA-155和患者预后的关系。结果：miR-NA-155在 HCC 癌组织中表达量显著高于癌旁组织（P＜0．01）；高 miRNA-155表达和 HCC 患者 TMN 分期（P＜0．05）、微血管侵犯（P＜0．05）密切相关。Kaplan-Meier生存分析显示，miRNA-15高表达的 HCC 患者平均生存期（16．07±1．52）个月，miR-NA-155低表达患者为（28．25±1．38）个月，差异有统计学意义（P＜0．05）。结论：miRNA-155在 HCC 患者中存在过表达，且与患者预后存在一定关系。%Objective:To observe the expression of micro-RNA-155 (miRNA-155)in tissues of hepatocellular cancer (HCC) and its relationship with clinical pathological characteristics and prognosis.Methods:Quantitative real-time reverse transcription poly-merase chain reaction (PCR)was used to detect the expression of miRNA-155 in the cancerous tissues and para-cancerous tissues of 77 patients with HCC,and the difference of miRNA-155 expression in HCC patients with different pathological characteristics was ana-lyzed.Kaplan-Meier survival curve was used to analyze the correlation between miRNA-155 and patients’prognosis.Results:miRNA-155 expression was evidently higher in cancerous tissues than that in para-cancerous tissues of HCC(P<0.01).High expression of miRNA-155 was in close association with TMN staging (P<0.05)and microvascular invasion (P<0.05)in patients with HCC.Kap-lan-Meier survival curve analysis showed that the average survival time was (16.07 ±1.52)months in HCC patients with high expres-sion of miRNA-155 and (28.25 ±1.38)months in those with low expression of miRNA-155,and there was significant difference (P<0.05).Conclusion:MiRNA-155 over-expresses in patients with HCC and it has certain relationship with patients’prognosis.