靶向REV3基因siRNA抑制人结肠癌细胞裸鼠移植瘤增殖作用的研究

摘要

目的 检测靶向REV3基因的siRNA对裸鼠体内人类结肠癌SW480和COLO-205细胞移植瘤增殖的影响.方法 采用脂质体-质粒转染技术构建REV3的siRNA,特异性地下调人类结肠癌细胞(SW480、COLO-205)中REV3基因的表达,通过实时荧光定量PCR(qRT-PCR)技术检测细胞的干扰效率,选择低表达效率具有统计学意义的细胞作为实验组细胞.将空白对照组、阴性对照组及实验组细胞分别注入到相应组裸鼠体内.通过观察裸鼠移植瘤的体积及裸鼠的体重来评价REV3 siRNA对人结肠癌细胞裸鼠移植瘤增殖的影响.结果 REV3基因的表达下调后,裸鼠体内移植瘤(SW480、COLO-205)实验组细胞成瘤的瘤体体积较对照组细胞成瘤的瘤体体积明显减小,实验组成瘤裸鼠的体重较对照组成瘤裸鼠的体重明显增加(P＜0.05);而阴性对照组和空白对照组的人结肠癌细胞裸鼠移植瘤的瘤体体积及裸鼠体重差别均无统计学意义(P＞0.05).结论 特异性的下调RE V3基因在结肠癌细胞(SW480、COLO-205)中的表达量,可能对裸鼠体内移植瘤的增殖具有一定的抑制作用.%Objective To investigate the effect of RE V3 siRNA induced profilication in human colon cancer cells,SW480 and COLO-205 in transplanted nude mice.Methods Via liposome along with plasmid transfection technology,Human colon cancer cells,SW480 and COLO-205 were transected to down-regulate the REV3 gene expression.The interfering efficiency was detected by quantitative real-time polymerase chain reaction (qRT-PCR).Cells which had the statistical meaning of down-expressed efficiency were thought of as experiment group.They were randomly divided into three groups,the blank control group,negative control group and the experimental group,which were injected into the nude mice.Results After down-regulate REV3 gene expression,Tumor volume was significantly decreased compared with the negative control group and blank control group (P＜0.05).Tumor in nude mice which were transplanted from the experimental groups cells (SW480,COLO-205),whose volume were significantly decreased.nude mice's weight in the experimental group increased significantly than that in control groups.Whereas,there was no statistical significance of the transplantation tumor volume and mice's weight between negative control group and blank control group (P＞0.05).Conclusion Down-regulated REV3 gene expression in human colon cancer cells (SW480 and COLO-205)might inhibit the cell proliferation which transplanted in nude mice.