维生素C和牛磺酸对铁过载小鼠心脏损伤的保护作用研究

摘要

Objective To investigate the protective effects of vitamin C and taurine on iron o-verload cardiomyopathy in mice. Methods A mouse model of myocardial iron overload was made by intraperitoneal injection of dextroglucose chalybeate. Iron-overloaded mice were intragastricly administrated with 150 mg ? kg-1 vitamin C (Asc group), 420 mg ? kg-1 taurine 75 mg ? kg-1 vitamin C plus 210 mg ? kg-1 taurine (Asc + Tau group) or 0. 5 mL normal saline (iron overload group). Control mice received 0. 5 mL normal saline only. The plasma creatine ki-nase (CK) and lactate dehydrogenase (LDH) activities were detected and myocardial malondial-dehye (MDA) content and superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) activities were measured in all mice. Results Plasma CK activities and myocardial MDA concentrations in Asc group,Tau group and Asc+Tau group significantly decreased compared with iron overload group(P<0. 05),but obviously increased compared with control group (P<0. 05). Moreover, vitamin C and taurine had a synergistic effect (P<00. 05). Plasma LDH activities in iron o-verload group were significantly higher than those in other groups(P<0. 05) ,but no differences were observed among other four groups(P>0. 05). Compared with iron overload group, plasma SOD activities significantly increased in Tau group, Asc + Tau group and control group (P< 0. 05) ,but there were no differences among the three groups (P>0. 05). Conclusion Vitamin C has a protective effect on myocardial damage induced by iron overload. In addition, vitamin C can synergize with taurine to improve iron overload cardiomyopathy in mice. (Tau group),%目的 探讨维生素C和牛磺酸对铁过载小鼠心脏损伤的保护作用.方法 采用腹腔注射右旋葡萄糖铁剂的方法,获得急性铁过载小鼠模型.急性铁过载小鼠再随机分组,分别给予维生素C(150 mg·kg-1)、牛磺酸(420mg· kg-1)、1/2维生素C(75 mg·kg-1)+1/2牛磺酸(210mg· kg-1)和0.5 mL生理盐水灌胃；对照组给予0.5 mL生理盐水灌胃,4周后,提取各组小鼠血浆测定血浆CK、LDH值,提取各组小鼠心脏测定心肌的MDA、GSH-PX、SOD值.结果 维生素C组、牛磺酸组和1/2维生素C+1/2牛磺酸组小鼠的血浆CK活性显著低于铁过载组(P＜0.05),但明显高于对照组(P＜0.05)；维生素C和牛磺酸对小鼠血浆CK活性的影响有协同作用(P＜0.05).铁过载组小鼠血浆LDH活性比其他4组小鼠血浆LDH活性明显升高(P＜0.05),其他4组之间小鼠血浆LDH活性差异无统计学意义(P＞0.05).维生素C组、牛磺酸组和1/2维生素C+1/2牛磺酸组小鼠心肌MDA含量比铁过载组明显降低(P＜0.05),但和对照组小鼠心肌MDA含量相比较差异无统计学意义(P＜0.05).维生素C和牛磺酸对降低小鼠心肌MDA的含量有协同作用(P＜0.05).铁过载组小鼠心肌SOD活性比牛磺酸组、1/2维生素C+1/2牛磺酸组和对照组小鼠明显降低(P＜0.05),牛磺酸组、1/2维生素C+1/2牛磺酸和对照组心肌SOD活性3组之间无差异(P＞0.05).结论 维生素C对铁过载引起的小鼠心脏损伤有保护作用；维生素C和牛磺酸在改善铁过载对小鼠所造成的心脏损伤方面有协同作用.