目的:探讨miR-181b在前列腺癌组织中的表达及miR-181b对前列腺癌PC-3细胞生物学功能的影响.方法:收集27例前列腺癌手术标本及30例正常前列腺组织标本,提取总微小RNA,应用实时荧光定量PCR技术检测miR-181b的表达情况.选取人前列腺癌细胞株PC-3细胞为研究对象,转染miR-181b ASO.应用实时荧光定量PCR技术检测转染miR-181b ASO PC-3细胞中miR-181b 的表达情况;流式细胞术检测转染miR-181b ASO PC-3细胞的凋亡变化情况;MTT实验及细胞生长曲线检测转染miR-181b ASO PC-3细胞增殖能力的影响;Transwell侵袭实验检测转染miR-181b ASO PC-3细胞侵袭能力的影响.结果:miR-181b在前列腺癌组织中高表达.转染miR-181b ASO 使PC-3细胞中miR-181b的表达降低;促进了PC-3细胞凋亡;miR-181b的表达降低导致前列腺癌细胞株PC-3增殖能力的减弱;miR-181b的表达降低导致前列腺癌细胞PC-3侵袭能力减弱.结论:miR-181b在前列腺癌组织中高表达,封闭前列腺癌细胞中miR-181b的表达,可以促进细胞凋亡及抑制细胞的增殖及侵袭,可能在前列腺肿瘤的基因治疗中起到积极作用.%Objective : To study the expression of miR - 181b in specimens of prostate cancer and to investigate the effects of miR - 181b on the biological behavior of prostate cancer PC - 3 cell line. Methods : issues from 27 cases of prostate cancer and 30 samples of normal human prostate were collected after surgical operation. Total miRNA was extracted and the relative expression of miR - 181b was quantified by Real - time PCR. miR - 181b ASO was transfected into prostate cancer PC - 3 cell. The expressions of miR - 181b in transfected and non - transfected cells were measured by Real - time PCR;The changes of cell apoptosis were measured by flow cytometry; MTT assay and growth curve were used to assess the effect of miR - 181b on cell proliferation; The changs of cell invasion abilities in vitro were detected hy Transwell chamber. Results : miR - 181b was up - regulated in prostate cancer comparing with normal prostate samples. miR - 181b was down - regulated after miR - 181b ASO was transfected into prostate cancer PC - 3 cell. Down - regulation of miR - 181b in PC - 3 cell resulted in inducing apoptosis of PC - 3 cell,inhibiting proliferation of PC - 3 cell and depressing the invasion of PC - 3 cell in vitro. Conclusion : miR - 181b was up - regulated in prostate cancer. Down - regulation of miR - 181b may act as a potential gene therapy of prostate cancer hy inducing apoptosis,inhibiting proliferation and depressing the invasion of PC - 3 cells.
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