应用X线照射后的Balb/c小鼠制备白血病模型的方法学研究

摘要

Objective :To explore the methods of preparing leukemia mouse model which were prepared with SPF Balb/c mouse irradiated by X ray and K562 cells that were transfered CFP and NeoR gene.Methods : Balb/c mice of experimental group were irradiated 2 or 3 Gy , then injected into log phase growth K562 ( GFP +/Neo + ) cells 2 ×106 by vena caudalis after 24hours,whereas the mice of control group were not treated.Results: The mouse of experimental group died within 30 and 24 days ; there was significant difference in survival time compared with that of normal control group( P ＜0.01) .The weight decreased significantly than that in normal control group ( P ＜ 0.05 ) .The incidence of leukemia in processed mice was lOO％ ,and had no natural relief.With the increase of irradiation dose, survive time of mice shorten gradually, the percentage of leukemia cells in peripheral blood and bone marrow increased gradually.The existence of GFP and NeoR gene was verified by FCM and PCR in peripheral blood , bone marrow , live and spleen.Conclusion : The leukemia mouse model can be made by infusing K562 cells by vena caudalis into Balb/c mouse after irradiated.%目的:探索用SPF级Balb/c小鼠经X线照射后,尾静脉注射转染GFP及NeoR基因的K562细胞株以制备白血病模型的方法.方法:实验组小鼠分别经X线照射2Gy和3Gy,24小时后取对数生长期的K562(GFP+/Neo+)细胞尾静脉注射2×106个/只.对照组不予特殊处理.结果:实验组在接种5-7天时发病,分别于30、24天内全部死亡;生存天数显著短于对照组(P<0.01).体重较对照组显著下降(P<0.05).小鼠的白血病发病率为100%,无自发缓解.随照射剂量的增加,小鼠的存活时间缩短,且外周血及骨髓中白血病细胞所占比例增加.流式细胞仪测定及PCR方法也证实了GFP+细胞和NeoR基因在外周血、骨髓、肝、脾中的存在.结论:Balb/c小鼠经照射后从尾静脉注射K562细胞株可以获得白血病模型.