黄芪甲苷对肝癌细胞增殖和侵袭能力的影响及其机制研究

摘要

目的：研究黄芪甲苷对肝癌细胞系 MeCC97- e 增殖和侵袭能力的影响。方法：MTT 法检测5、10、20、40、80、100μg/ ml 不同终浓度和24、48、72h 作用时间下黄芪甲苷对 MeCC97- e 细胞增殖的影响。Tran-swell 侵袭实验检测100μg/ ml 黄芪甲苷处理48小时对 MeCC97- e 细胞侵袭能力的影响。选择100μg/ ml 黄芪甲苷处理48小时作为处理条件，实时定量 PCR、Western blot 检测黄芪甲苷处理后 MeCC97- e 细胞 MMP%Objective:To investigate the anti - Proliferation and anti - invasion effect of astragaloside IV on liver cancer cell line MeCC97 - e in vitro. Methods:MeCC97 - e cells were exPosed to different concentrations of astra-galoside IV(A - IV)for 24h,48h and 72h resPectively. The Proliferation was detected by 4 - methyl - teerazolium (MTT). Transwell assay was aPlied to detect the inhibitory effect of A - IV on MeCC97 - e cell invasion. MeCC97- e cells were treated by 100μg/ ml A - IV for 48 hours. Then the exPressive changes for matrix metalloProteinase -2,9(MMP - 2,9)mRNA and Protein in MeCC97 - e cells were detected by real - time PCR and Western bolt re-sPectively. The contents of MMP - 2,9 in culture suPematant of MeCC97 - e cells were measured by gelatin zymog-raPhy. Results:MTT showed that A - IV had no significantly inhibitoty effect on MeCC97 - e cells with any concen-trations and times. The result of Transwell assay showed that A - IV could inhibit the invasion of MeCC97 - e cells in vitro. The exPressions of MMP - 2,9 mRNA and Protein reduced after treatment by 100μg/ ml A - IV for 48 hours. And the contents of MMP - 2,9 in culture suPematant of MeCC97 - e cells reduced. Conclusion:A - IV has no ob-vious inhibitory effect on the Proliferation of MeCC97 - e cells,but a strong inhibitory action on the invasion of Me-CC97 - e cells in vitro.