儿童急性髓系白血病免疫表型特点分析

摘要

目的:分析儿童急性髓系白血病(AML)免疫表型特征,探讨其临床意义.方法:采用四色流式细胞术CD45/SSC双参数散点图设门方法,对127例儿童AML患者幼稚细胞进行免疫表型检测,对抗原表达情况进行分析.结果:在127例儿童急性髓系白血病患者中,髓系特异性抗原CD33、CD13和CD117的表达最常见,分别达95.3%、90.6%、90.6%.造血干/祖细胞抗原CD34、HLA-DR、CD38阳性率分别达53.5%、71.6%和97.6%.有65.4%的病例伴有淋系抗原的表达,其中以CD56的表达最常见占38.6%,其次为CD7占21.3%.有淋系抗原表达(LymAg+)患者早期抗原CD34和HLA-DR抗原表达阳性率明显高于无淋系抗原表达(LymAg-)患者(P<0.05).结论:免疫分型对儿童AML的诊断和不同亚型鉴别具有重要意义.%Objective:To investigate the immune phenotypic characteristics of childhood acute myeloid leukemia(AML) and its clinical significance.Methods:The immune phenotype in 127 cases with childhood AML were detected by four color flow cytometry with CD45/SSC double parameters scatter plot setting as door,and then the antigentic expressions were analysed.Results:In 127 patients with childhood AML,the Myeloid antigen of CD33,CD13,CD117 showed highly expressed.The positive rates were 95.3%,90.6%,90.6%.Hematopoietic stem/progenitor cell marker antigen CD34,HLA-DR and CD38 were expressed in AML with the rates of 53.5%,71.6% and 97.6%.The lymphoid antigen was positive in 65.4%of cases,with the highest frequency of CD56(38.6%)followed by CD7(21.3%).The expression of CD34+ and HLA-DR+ patients in LymAg+ group were significantly higher than LymAg-group(P<0.05).Conclusion:The immunophenotyping played an important role in diagnosis and classification of childhood AML.