FAK和Src在甲状腺乳头状癌中的表达及其在肿瘤侵袭转移中的临床意义及机制研究

摘要

目的：探讨黏着斑激酶（FAK）和Src在甲状腺乳头状癌侵袭转移中的临床意义。方法：利用免疫组化检测FAK和Src在100例甲状腺乳头状癌组织中的表达，并分析其与患者临床病理参数的关系。利用Western blot以及qRT－PCR检测FAK和Src在甲状腺癌细胞株中与上皮间质转化（EMT）的相关性。结果：FAK与甲状腺乳头状癌的年龄（P＝0．000）、T分期（P＝0．176）、N分期（P＝0．000）、M分期（P＝0．000）、临床分期（P＝0．038）、局部复发（P＝0．000）、淋巴结侵袭（P＝0．014）以及与患者较低生存期（P＜0．0001）密切相关；Src与甲状腺乳头状癌的N分期（P＝0．000）、M分期（P＝0．002）、淋巴结侵袭（P＝0．000）、包膜侵袭（P＝0．029）以及患者的较低生存期（P＜0．0001）密切相关。并且高表达FAK与Src的细胞其N－cadherin、Vimentin的表达增高，而E－cadherin的表达降低。结论：FAK以及Src与甲状腺乳头状癌的侵袭转移密切相关，并可能通过EMT的方式促进其侵袭和转移。%Objective:To evaluate the clinical significance and mechanism research of FAK and Src expression in the process of invasion and metastases in papillary thyroid carcinoma (PTC ).Methods:Immunohistochemistry was used to detect FAK and Src expression in 100 PTC tissues.The relationship between FAK and Src expression and the clinical pathological parameters of patients including gender,age,TNM staging,clinical stage,local recurrence,lym-phatic invasion,capsular invasion and vascular invasion were analyzed.Western blot and qRT-PCR were performed to detect the expression of FAK and Src and its correlation with epithelial-to -mesenchymal transition(EMT)in PTC cell lines.Results:FAK expression in PTC was correlated strongly with age (P=0.000),T classification(P=0.176),N classification(P=0.000),Mclassification(P=0.000),clinical stage(P=0.038),local recurrence(P=0.000),lymphatic invasion(P=0.014)and worse survival of PTC(P<0.000 1).Src expression in PTC was corre-lated strongly with N classification(P=0.000),M classification(P=0.002),lymphatic invasion(P=0.000),cap-sular invasion(P=0.029)and worse survival of PTC(P<0.000 1 ).Furthermore,high expressions of FAK and Src were correlated strongly with high expression of N-cadherin and Vimentin and low expression of E-cadherin in PTC cell lines.Conclusion:Our findings demonstrate that FAK and Src high expression links closely with EMT and thus may contribute to tumor invasion and metastases.