原癌基因Bmi-1 B细胞抗原表位及HLAⅠ类限制性CTL表位的生物信息学分析

摘要

Objective:To predict the HLA Ⅰ restricted CTL epitopes derived from proto-oncogene Bmi-1.Meth-ods:The linear B cell epitopes and conformational B cell epitopes of proto-oncogene Bmi-1 were predicted by El-lipro program.The HLA I restricted CTL epitopes of proto-oncogene Bmi-1 were predicted by ProtParam,NetCTL methods.Results:B cell epitopes analysis revealed that Bmi-1 has 6 potential linear B epitopes and 8 conformational B epitopes.Combined with peptide HLA class Ⅰ binding,proteasomal C terminal cleavage and TAP transport efficien-cy,the NetCTL program predicts that multiple HLA class Ⅰ restricted CTL epitopes were present in the proto-onco-gene Bmi-1.Conclusion:The B cell epitopes and HLA class Ⅰ restricted CTL epitopes were predicted by using of multiple procedures,which may lay the foundation for the further research on immunotherapy for targeting Bmi-1.%目的:预测原癌基因Bmi-1(B-cell-specific Moloney murine leukemia virus insertion site 1)的B细胞抗原表位及HLA I类限制性细胞毒性T细胞(cytotoxic T cell)表位.方法:采用Ellipro(http://tools.immu-neepitope.org/ellipro/)预测Bmi-1蛋白中可能存在的线性B细胞表位和构象B细胞表位;采用ProtParam、NetCTL等软件和方法预测Bmi-1中可能存在的HLA I类限制性CTL表位.结果:Bmi-1蛋白中含有6个线性B细胞表位和8个构象B细胞表位;结合HLA结合力、蛋白酶体切割效率和TAP转运效率,经过NetCTL程序预测表明:针对不同的HLA I类分子,原癌基因Bmi-1中都存在多个HLA I类分子的限制性CTL表位.结论:综合运用多个程序预测了原癌基因Bmi-1中的B细胞抗原表位及HLA I类分子的限制性CTL表位,为下一步开展针对Bmi-1的免疫靶向治疗等研究打下了基础.