过表达GDF15对肺腺癌细胞A549增殖、迁移和侵袭能力的影响

摘要

Objective:To explore the effects of GDF15 on the biological behavior of the human lung adenocarcino-ma cell line A549,in order to provide a new target for the prevention and treatment of lung cancer.Methods:The ex-pression of GDF15 was up-regulated by transfection with plasmid in A549 and identified by RT-PCR and Western-blotting.The effects of GDF15 on the biological behavior of A549 cells were observed by MTT assay,colony-form-ing assay and Transwell based methods.Results:A549-GDF15 cell line and A549-neo cell line(A549 transfected by neo empty vector)were successfully constructed.Compared with A549-neo,the overexpression of GDF15 can in-hibit the proliferation,migration and invasion ability of A549 cells.Conclusion:This study finds a negative correlation between GDF15 expression and the development and metastasis of lung cancer.%目的:探究GDF15在体外对人肺腺癌细胞株A549生物学行为的影响,为肺癌防治提供新的靶点.方法:通过质粒转染的方法,构建GDF15过表达和neo空载体的A549细胞株,运用MTT法、平板克隆和Tran-swell的实验方法,观察过表达GDF15对A549细胞株生物学行为的影响.结果:成功构建 GDF15过表达的A549-GDF15细胞株和neo空载体的A549 -neo细胞株.与空载体细胞株相比,过表达GDF15能够抑制A549细胞的增殖、迁移和侵袭能力.结论:GDF15在体外可抑制肺癌细胞的增殖及迁移.