肝脏Kupffer细胞摄取清除腺病毒载体的机制

摘要

Adenovirus (Ad) Vectors are promising tools for gene therapy because of their high transduction efficiency and wide tropism. However, when Ad is administered systemically by intravenous (I. V. ) in jection, the efficiency of gene delivery is greatly impaired due to rapid removal of virions from the circulation by the reticulo-endothelial system (RES) .particularly by Kupffer cells (KCs)in the liver,which is a major barrier to systemic adenovirus (Ad) gene therapy. This paper reviews some mechanisms which are thought to contribute to uptake and clear of Ad in the liver by KCs. It is helpful to provide the clinical reference for realizing transgenic lasting expression of adenovirus vector in gene therapy.%腺病毒载体因其具有广泛趋向性、高效率表达目的基因等优点而成为表达和传递治疗基因的主要候选者.但是,由于它可以快速的经循环系统到达网状内皮系统(特别是Kupffer细胞)被摄取和清除,只有少数碎片能到达靶器官,因此这也成为了腺病毒作为载体应用于基因治疗的最主要障碍.该文综述了Kupffer细胞摄取、清除腺病毒这一过程可能存在的机制,为实现腺病毒裁体转基因持久表达提供临床参考.