西安地区男性尿道感染人乳头瘤病毒基因亚型分析

摘要

目的 探讨西安地区男性尿道感染人乳头瘤病毒(human papillomavirus,HPV)基因亚型特点.方法 采用PCR-反向斑点杂交法(reverse dot hybridization assay,RDB)检测2015～2016年陕西省人民医院皮肤科478例男性尿道分泌物中18个HPV基因型,低危型HPV包括HPV6,11,43和高危型HPV包括HPV16,18,31,33,35等亚型.结果 478例受检者中HPV感染率为44.98％(215/478),单一HPV亚型、两种HPV亚型、三种HPV亚型、四种HPV亚型和五种HPV亚型的感染率分别为32.85％(157/478),8.79％(42/478),2.09％(10/478),0.42％(2/478)和0.84％(4/478);低危型HPV亚型和高危型HPV亚型累计检出率分别为40.17％和22.38％,其中检出率前五位HPV亚型为HPV6(24.69％),HPV11(12.13％),HPV16(5.65％),HPV43(3.35％)和HPV52(2.30％),其他亚型检出率介于0％～2.09％;在同年龄段受检者中HPVs感染模式均以单一感染为主;21岁以上的受检者中主要HPV亚型(HPV6,11,16,43,52和66)检出率差异均有统计学显著性意义(x2=12.879～109.7,P=0.000～0.025).结论 西安地区男性尿道感染的HPV亚型以HPV6,11,16,43和52为主,为研究男性感染HPV的流行病学提供数据和资料.%Objective To investigate the infection of HPV subtypes in male urethra in Xi'an area.Methods The eighteen subtypes of HPV DNA were detected in 478 cases of male patients by PCR-reverse dot hybridization assay (RDB) including low risk subtypes (HPV6,11,43) and high risk subtypes (HPV16,18,31,33) during 2015～2016.Results The total infection rate of HPV subtypes in 478 subjects was 44.97％ (215/478).The percents of subjects infected by one subtype,two ones,three ones,four ones and five ones were 32.85％ (157/478),8.79％ (42/478),2.09％ (10/478),0.42％ (2/478) and 0.84％ (4/478),respectively.The detection rates of low risk subtypes and high risk ones were 40.17％ and 22.38％ in which the most common subtypes were HPV6 (24.69％),HPV11 (12.13％),HPV16 (5.65％),HPV43 (3.35％) and HPV52 (2.30％) and those of others were from 0％ to 2.09％.The infection pattern of HPV subtypes gave priority to one subtype infection in all age groups.There was statistically different between H PVs (6,11,16,43,52,66) in twenty years or older subjects (x2 =12.879～109.7,P=0.000～0.025).Conclusion The majority of HPV subtypes detected in male urethra were HPV6,HPV11,HPV16,HPV43 and HPV52 in Xi'an area which provided epidemiological data for HPV infection in males.