滁州地区女性人乳头瘤病毒感染状况及基因亚型分析

摘要

目的:了解滁州地区女性人乳头瘤病毒(HPV)感染率、基因亚型及年龄特点.方法:采用PCR-反向点杂交法技术,对2012年滁州地区7家医院送检的2 554例女性患者宫颈分泌物或脱落细胞进行HPV 23种基因型检测.结果:2 554例患者中共检出HPV阳性557例,感染率21.81%,基因型82 未检出.单一感染率16.33%,双重感染率3.92%,三重以上感染率1.57%.阳性患者中高危型以HPV16、58 和52 型为主,低危型主要以HPV43、81 型为主.23 种基因型中感染率前5 位是HPV16、58、43、52、81.感染的高峰年龄主要分布在19 ~30 岁;HPV16 感染率3.99%,HPV16 混合其他亚型感染率1.61%;HPV16感染主要分布在61~70岁;单一和三重以上感染的高峰年龄分布在19~30岁,双重感染高峰年龄分布在51~60岁.结论:滁州地区女性HPV感染以HPV16、58和43为主要的流行亚型,且HPV感染呈上升趋势,发病年龄趋于年轻化.%Objective:To investigate the infection rate of human papillomavirus(HPV),its genotype and age characteristics among women in Chuzhou area. Methods:Twenty-three genotypes in cervical secretion or castoff cells in 2 554 women from Chuzhou area were detected using PCR-reverse dot blot. Results:Among 2 554 patients,557 cases with positive HPV infection were detected,the infection rate of which was 21. 81%,and the 82 genotype was not detected. The single infection,dual infection and more than triple infection rates of HPV were 16. 33%,3. 92% and 1. 57%,respectively. HPV16,58 and 52 were the most prevalent types in the positive cases with high risk genotype,HPV43 and 81 were the most prevalent types in the positive cases with low risk genotype. Among 23 genotypes,the infection rates of HPV16,58,43,52 and 81 were the first five in turn. The peak age of infection was mainly distributed in 19 to 30 years old women. The infection rates of HPV16 and HPV16 mixed with other subtypes were 3. 99% and 1. 61%,respectively. The peak ages of the infection of HPV16,single infection and more than triple infection of HPV,and dual infection were 61 to 70,19 to 30 and 51 to 60 years old. Conclusions:The HPV16,58 and 43 in HPV infection among women from Chuzhou area are the mainly popular subtypes,the HPV infection is on the rise,and the age of onset tends to be younger.