温度响应的细菌生理生化特性调控研究进展

摘要

In this paper, the physiological and biochemical characteristics of bacteria in response to temperature changes, how do DNA, RNA and protein molecules in cells as temperature sensing elements respond to temperature changes, and the mechanisms by which cells regulate physiological and biochemical characteristics, were summarized.The elements, structure and controlling mode in typical two-component system (TCSs) responsible for response to temperature were focused, such as Cor S/CorR TCSs regulating expression of cellular genes response to temperaturechange in Pseudomonas aeruginosa PG1480, Des K/DesR TCSs regulating the expression of fatty acid desaturase des gene respond to temperature change in Bacillus subtilis, and Lot S/LotR TCSs found recently, regulating the expression of low temperature-responsive protease in Stenotrophomonas maltophilia FF11. Meanwhile, the action modes of c-diGMP as second messenger in TCSs control process respond to temperature were reviewed. The hot issues, key technologies and the suggested research strategies were presented.%针对细菌对温度变化响应呈现的生理生化特性变化, 总结了细胞内作为温度感应元件DNA、RNA及蛋白质分子如何响应温度变化, 以及细胞调控生理生化特性的机制.重点介绍了典型的温度响应双组分系统的组成、结构及调控方式, 如铜绿假单胞菌PG1480的CorS/CorR双组分系统响应温度的刺激调控细胞基因的表达, 枯草芽胞杆菌的DesK/DesR双组分系统响应外界温度变化, 调节编码脂肪酸去饱和酶基因des的表达, 以及在嗜麦芽单胞菌中发现的LotS/LotR双组分系统, 调控低温响应蛋白酶的表达.同时总结c-di-GMP作为第二信使参与温度响应双组分调控的机制;提出研究的热点问题和关键技术以及建议的研究策略.