碱性羟胺、弱碱和乙酸水解奇异变形杆菌LPS抗原及其活性的影响

摘要

采用碱性羟胺、弱碱、乙酸水解奇异变形杆菌LPS抗原，研究不同方式对小鼠活性的影响。检测结果表明，与水解前对比三种方式均能降低LPS抗原内毒素含量，即从2500×104 EU／mL降低至（250～2．5）×104 EU／mL；热源性检测表明对家兔体温几乎无影响；但免疫原性结果表明 ED50 LPS ＜ED50 Acid-LPS＋Al（OH）3＜ED50 Alkaline-LPS＋Al（OH）3＜ED50 HA-LPS＋Al（OH）3，毒性结果为LD50 HA-LPS ＜LD50 Alkaline-LPS ＜LD50 LPS ＜LD50 Acid-LPS。因此，经过乙酸（体积分数2％）或0．1 mol／L弱碱（体积分数2％）水解，能够显著降低内毒素含量及热源性，并保持其生物活性，使之成为有效的候选疫苗组分，用于预防或治疗大面积烧伤及术前或术后感染。%Proteus mirabilis LPS antigen was hydrolyzed adopting alkaline hydroxylamine , mild alkaline and acetic acid to study the effect of its activity on mice .The determination results showed that as compared with the pre-treat-ment, three methods could reduce endotoxin levels of LPS antigen from 2 500 ×104 EU/mL to (250~2.5) ×104 EU/mL.Pyrogenic properties showed almost no significant effects on the rabbit body temperature .However , immunoge-nicity results showed that ED50 LPS