82例系统性红斑狼疮5种自身抗体及补体结果分析

摘要

目的 探讨系统性红斑狼疮(SLE)患者血清抗核抗体(ANA)、抗双链DNA抗体(dsDNA)、抗史密斯抗体(Sm)抗体、抗核糖体P蛋白抗体(Rib.P.P)、抗核小体抗体(AnuA)、补体C3和C4阳性分布特点及临床价值.方法 对82例SLE、41例结缔组织病(CTD)、33例干燥综合征(SS)、61例类风湿性关节炎(RA)患者与50例健康对照组分别采用欧蒙间接免疫荧光法检测血清ANA和抗-dsDNA;欧蒙免疫印迹法检测其他自身抗体;免疫散射比浊法测定血清C3和C4的浓度.结果 SLE组ANA、抗-dsDNA、抗-Sm抗体、抗-Rib.P.P和AnuA的阳性率和其他疾病组和健康对照组比较,差异有统计学意义(P＜0.05).SLE组C3与CTD和SS组C3比较,差异无统计学意义(P＞0.05),与RA组和健康组比较,差异有统计学意义(P＜0.05).SLE组C4与CTD组比较,差异无统计学意义(P＞0.05),与SS、RA组及健康组比较,差异有统计学意义(P＜0.05).结论 联合检测血清ANA、抗-dsDNA、抗-Sm抗体、抗-Rib.P.P、AnuA、和补体3及C4,可提高SLE的早期诊断的准确性.%Objective To study the clinical value of detecting serum antinuclear antibodies (ANA) ,anti - dsDNA ,anti - Sm, antirn- ribosomal phosphoprotein antibodies( anti - Rib. P. P) , antinucleosome( AnuA) in SLE early diagnosis. Methods The serum levels of ANA, anti - dsDNA in 82 cases of SLE patients and 41 cases of CTD ,33 cases of SS ,61 cases of RA and 50 cases of healthy controls were detected by indirect immunofluorescence assay. The serum levels of other auto - antibodies in the same individuals were detected by Europern- Mongolia immunity print. The serum levels of C3 and C4 were measured by immune scattering turbidimetrytest. Results The difference in AN A, anti - dsDNA ,anti - Sm,anti - Rib P. P and AnuA between patients with SLE ,CTD,SS and healthy controls was compared (P < 0. 05 ) . There was no difference in C3 between SLE, CTD and SS( P > 0. 05 ) , and there was difference in C3 between SLE, RA and health-y controls(P<0.05). There was no difference in C4 between SLE and CTD(P >0.05) ,and there was difference in C4 between SLE,SS, RA and healthy controls (P <0. 05). Conclusion Combined detection of AN A, anti - dsDNA,anti - Sm.anti - Rib P. P, AnuA,C3 and C4 in serum is helpful for increasing the accuracy of SLE early diagnosis.