心力衰竭大鼠外周血生长分化因子-15及其心肌组织中mRNA表达水平

摘要

目的 研究心力衰竭(heart failure,HF)大鼠外周血生长分化因子-15(growth differentiation factor-15,GDF-15)含量变化及心肌组织GDF-15mRNA表达与心力衰竭发生发展关系.方法 结扎大鼠左前降支制备心力衰竭模型,采用酶联免疫方法与实时荧光定量方法检测外周血中GDF-15及心肌组织GDF-15mRNA表达;彩色多普勒检测实验大鼠心功能.结果 随着病程进展,HF大鼠外周血液GDF-15含量1452.03±76.88pg/ml高于正常大鼠外周血GDF-15含量26.53±34.06pg/ml,HF大鼠心肌组织GDF-15mRNA亦随之增高,而假手术组则表达降低,差异有统计学意义;心功能(Left ventricμlar ejection fraction) EF值随着外周血及心肌组织中GDF-15表达水平增高而降低.结论 外周血中GDF-15含量变化及心肌组织mRNA表达调控与HF发生发展密切相关,可以作为预测心力衰竭进展新的生物标志物.%Objective To research the expressing level of GDF - 15 in peripheral blood and its mRNA in the myocardial tissue in rats in the HF development. To explore the corelation between the GDF - 15 expressing level and the development of HF in rat. Methods The establishment of rat HF model by ligaturing left descending branch of rat coronary artery : detecting GDF - 15 expression level in peripheral blood by enzyme linked immunosorbent assay with real - time fluorescence quantitative method, observe GDF - 15 mRNA in myocardial tissue by PCR, and detect the rat cardiac function by Color Doppler. Results With the development of this HF, the level of peripheral blood GDF - 15 of HF group rats was 1452. 03 -6. 88pg/ml, while it was 26. 53 -4. 06pg/ml in normal control group rats . GDF - 15 mRNA expression in myocardial tissue shows heigher and lower level in HF group rats and normal control group rats .respectively , and the difference had statistical significance. EF was reduced accompanied with the increasing expression of peripheral blood GDF -15 and tissue GDF - 15 mRNA in HF group rats. Conclusion The changes of peripheral blood GDF - 15 and the myocardial tissue GDF - 15 mRNA expression level has a close relationship with the occurrence and development of HF. The results shows that GDF - 15 is a new potential biological marker to predict heart failure progress.