白细胞介素 10-1082G/A 位点单核苷酸多态性与膀胱癌的相关性

摘要

To investigate the relationship between polymorphism at - 1082G/A in the promoter region of the IL - 10 gene and the risk of bladder cancer. Methods In a hospital based case - control study for the Chinese population in Zhejiang, a total of 400 patients with bladder cancer and 400 age - and sex - matched healthy controls subjects were collected. We analyzed the genotypes of IL - 10 - 1082G/A by allele specific PCR amplification (AS - PCR) and DNA sequencing, and determined their association with bladder cancer risk and clinical stage. Results The genotype distribution and allele frequency of IL - 10 - 1082G/A gene had significant differences between patient group and control group(x2 = 8. 375, P < 0.05 ;x2 = 8.322, P <0. 01). Compared to the individual carrying AA genotype, the risk of bladder cancer with GC genotype and GA genotype increased 2.055 times( OR =2.055, 95% CI: 1.252 -3.374). Compared to the individual carrying allele A, the risk of bladder cancer with allele G increased 1. 990 times( OR = 1. 990, 95% CI: 1. 237 - 3. 203 ) . We did not observe any association between the genotypes of IL - 10 - 1082G/A with the clinical stage of bladder cancer. Conclusion IL - 10 - 1082G/A gene polymorphism may be a risk factor for bladder cancer and it may serve as a molecular marker for detecting bladder cancer.%目的 探讨白细胞介素-10(IL-10)基因启动子区域- 1082G/A位点单核苷酸多态性与膀胱癌危险性的关系.方法 选取浙江汉族人群中膀胱癌患者400例为病例组,400例健康体检者为对照组,以等位基因特异性扩增(allele specific PCR amplification,AS - PCR)结合DNA测序方法,检测病例组和对照组的IL -10 -1082G/A基因型,比较不同基因型与膀胱癌危险性以及膀胱癌临床分期之间的关系.结果 病例组和对照组的IL - 10 - 1082G/A位点基因型分布和等位基因频率之间差异有统计学意义(x2=8.375,P＜0.05;x2=8.322,P＜0.01)；AA基因型的个体与携带G等位基因型的个体(GG型和GA型)相比,携带等位基因A的个体与携带等位基因G的个体相比,其患膀胱癌危险性分别增加2.055倍和1.990倍(OR=2.055,95％ CI:1.252 ～3.374; OR=1.990,95％ CI:1.237 - 3.203);IL - 10 - 1082G/A位点基因型与膀胱癌的临床分期无直接相关性.结论 IL - 10 - 1082G/A多态性与膀胱癌危险性相关,该位点SNP检测可作为膀胱癌可能的分子标志物.