白细胞介素-23及其受体基因rs11209032位点单核苷酸多态性与强直性脊柱炎发病的相关性分析

摘要

目的：探讨白细胞介素-23（IL-23）、IL-23受体（IL-23R）基因rs11209032位点单核苷酸多态性与强直性脊柱炎（AS）的关系。方法选取五邑地区汉族人群AS患者132例和同期健康体检者102名，采用酶联免疫吸附试验（ELISA）法检测血清中IL-23的表达水平，采用TaqMan探针对IL-23R基因rs11209032位点进行聚合酶链反应（PCR）分型。结果 AS患者外周血IL-23表达水平显著高于对照组（P＜0.05）；在五邑地区汉族人群中，AA，AG， GG基因型频率在AS中分别为5.3%，91.7%，3.0%，与对照组分布（20.6%，65.7%，13.7%）相比，差异有统计学意义（χ2=24.63，P＜0.05），AS患者和对照组中rs11209032位点A等位基因频率（51.1%，53.4%）和G等位基因频率（48.5%，46.6%）的分布差异无统计学意义（χ2=0.243，P＞0.05）；其各个基因型组的红细胞沉降率和C反应蛋白水平差异无统计学意义（P＞0.05）。结论 IL-23R单核苷酸多态性rs11209032与本地人群AS发病有一定的相关性。%Objective To investigate the association between interleukin (IL)-23, interleukin-23 receptor(IL-23R) gene rs11209032 single nucleotide polymorphism (SNP) and ankylosing spondylitis (AS) in Chinese Han patients. Methods One hundred and thirty two patients with AS and 102 healthy donors were studied , enzyme linked immunosorbent assay (ELISA) was used analyze the levels of IL-23, The genotypes of IL-23R SNP was detected by TaqMan probe assays. Results The levels of the peripheral blood IL-23 were significantly higher in AS patients than those in normal controls (P<0.05);The rs11209032 genotypes in AS patients consisted of homozygote A/A (5.3%), A/G (91.7%) and G/G(3.0%), while The rs11209032 genotypes in the controls were composed of A/A(20.6%), A/G(65.7%) and G/G (13.7%). The significant difference was found in the distribution of rs11209032 genotypes between these two groups (χ2=24.63,P<0.05). But the frequency of rs11209032 allele in AS patients was not significantly changed when compared with the control group (χ2=0.243, P>0.05). Conclusion There may be existence association between the IL-23R gene rs11209032 SNP and ankylosing spondylitis in local area population .