目的 探讨灯盏花素对链脲佐菌素(STZ)诱导的糖尿病大鼠肾皮质RAGE表达水平的影响.方法 雄性SD大鼠27只,随机分为3组.STZ腹腔注射建立糖尿病模型后,糖尿病灯盏花素治疗组(DD组)每日给予灯盏花素[20mg/( kg·d)]灌胃,8周后取肾脏组织行半定量RT - PCR检测RAGE mRNA的表达水平,与正常对照组(C组),糖尿病安慰剂组(D组)比较.结果 成模8周时,糖尿病大鼠的体重、血糖与正常组相比有显著性差异(p＜0.01),DD组与D组的血糖及体重无显著性差异(P＞0.05)；DD组、D组和C组的RAGE OD值/β- actin OD值分别为0.452±0.208,0.769±0.104,0.218±0.032,各组间RAGE表达有显著性差异(P＜0.01).结论 糖尿病大鼠肾皮质RAGE mRNA表达明显高于正常对照组.灯盏花素能下调肾皮质RAGE的表达水平,对糖尿病肾病具有一定治疗作用.%Objective To determine whether erigeronbreviscapus affects expression of RAGE mRNA in renal cortex of streptozotocin - induced diabetic rats. Methods We induced diabetic rats by an intraperitoneal injection of streptozotocin (STZ) in SD rats. The criterion of diabetes diagnosis was the consecutive blood glucose levels 16. 7mmol/L. Totally 27 male SD rats were randomly divided into 3 groups:diabetes adding erigeronbreviscapus group ( DD group, intragastric administration erigeronbreviscapus 20mg/( kg · d) and diabetes group ( D group) and normal control group ( C group). The body weight and blood glucose were measured every two weeks. Totally 8 weeks later, all rats were killed and the expression of RAGE was semiquantified in renal cortex by reverse transcription - polymeraae chain reaction ( RT - PCR) , and renal morphology was evaluated respectively. Results The RAGE expression was increased in renal cortex of diabetic rals. RAGE/p - actin ratio of diabetic group was significantly higher than that of the control (P <0.01). After treatment with erigeronbreviscapus, RAGE expression decreased significantly. Conclusion The high expression of RAGE may participate in the renal damage of diabetic rats. Erigeronbreviscapus has no effect on the level of serum glucose, but significantly down - regulate RAGE expression in renal. It may have some renal protective effect on diabetic nephropathy, partly through inhibition of excessive expression of RAGE in renal cortex.
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