重组人脑利钠肽对大鼠液体胃排空和小肠传输功能的影响

摘要

目的 探讨重组人脑利钠肽对大鼠液体胃排空和小肠传输的影响.方法 将72只健康雄性Wistar大鼠随机分为治疗组和对照组,分别每日腹腔注射1次重组人脑利钠肽和生理盐水,1周后用葡萄糖酚红溶液灌胃,分别于灌胃后10、20、30 min处死大鼠,切取胃和小肠近、中、远各段留取内容物后,使用分光光度计测定胃肠各段酚红残留率.取治疗组和对照组大鼠各5只在灌胃前2h分别腹腔注射奥美拉唑,灌胃后10 min处死大鼠,测定胃内酚红残留率.结果 灌胃后10、20、30 min治疗组胃内酚红残留率分别为59.4％±1.9％、42.2％±1.7％和36.9％±2.7％,对照组为50.2％±2.0％、29.8％±1.1％和21.7％±1.3％,治疗组胃内酚红残留率高于对照组(P＜0.05)；治疗组和对照组小肠近段与中段酚红残留率差异无统计学意义；治疗组远段小肠酚红残留率分别为0.2％±0.2％、0.4％±0.1％和2.1％±1.9％,对照组分别为2.7％±0.6％、5.9％±1.4％和7.8％±2.1％,两组比较治疗组酚红残留率低于对照组(P＜0.05)；奥美拉唑预处理灌胃后10 min治疗组较对照组胃内酚红残留率增多,分别为60.7％±8.1％和49.8％±6.4％(P＜0.05),与未给予奥美拉唑预处理组比较,差异无统计学意义.结论 重组人脑利钠肽可使大鼠液体胃排空减慢,小肠传输延迟.%Objective To assess the effects of recombinant human brain natriuretic peptide (rhB-NP) on gastric emptying and gastrointestinal transit of liquid in rats. Methods The male Wistar rats were randomly divided into rhBNP treatment group and control group. The rats in treatment group received intraperitoneal injection of rhBNP (15 /μg/kg, once daily), while the rats of control group only received an equal volume saline. They were gavage fed (1.5 mL) next week with phenol red in 5% glucose solution and sacrificed 10, 20, 30 min later. Dye recovery in the stomach, proximal, mid, and distal small intestine was obtained by spectrophotometry. To rule out the effect of rngastric secretion changes, other group was omeprazole-pretreated before gavage fed. Results Gastric dye recovery of the rhBNP treatment group was greater compared to the control (59.4%±1.9% vs 50.2%±2.0%, 42.2%±1.7% vs 29.8%±1.1% and 36.9%±2.7% vs 21.7%±1.3%) in all subsets respectively at 10, 20, 30 min (P <0.05). There were no differences in proximal and mid small intestine retention between rhBNP treatment group and control group in all subsets at 10, 20, 30 min. While distal small intestine dye recovery of the rhBNP treatment group decreased compared to that of the control (0.2%±0.2% vs 2.7%±0.6%, 0.4%±0.1% vs 5.9%±1.4%, and 2.1%±1.9% vs 7.8%±2.1%) in all subsets respectively at 10, 20, 30 min (P <0.05). Omeprazole-pretreated did not alter this phenomenon. Gastric dye recovery of the rhBNP treatment group was greater compared to the control (60.7%±8.1% vs 49.8%±6.4%) at 10 min(P <0.05). Conclusion The rhBNP delays the gastric emptying and gastrointestinal transit of liquid in rats.