喜树叶中喜树碱和10-羟基喜树碱的HPLC分析

摘要

建立了同时测定喜树叶片中喜树碱含量的高效液相色谱分析方法.采用sinochrom ODS C18分析柱(250 mm×4.6 mm BP,5.0μm),以甲醇/水为流动相,检测波长为266 nm,梯度洗脱程序为:在前6 min流动相甲醇-水的体积比由40%线性增加至50%,并保持恒定2 min,在随后的32 min甲醇-水的体积比线性增至70%,在40 min时停止该程序.喜树碱在低、中、高3个量的平均加样回收率分别为99.66%,100.3%,99.97%.10-羟基喜树碱在低、中、高3个量的平均加样回收率分别为99.87%,99.98%,100.4%.对不同季节的喜树嫩叶和成熟叶进行了喜树碱和10-羟基喜树碱含量分析,结果表明喜树碱和10-羟基喜树碱的含量变化呈现明显的相关性.%An HPLC method for the simultaneous determination of CPT and HCPT in Camptotheca acuminata Decne.leaves was established.Chromatography was performed with a sinochrom ODS column,monitored at 266 am and the mobile phase was gradient H2O/CH3 OH.The solvent gradient program was as follows:methanol-water increased linearly from 40% to 50% at the first 6 min and retained for 2 min with 50%,then increased linearly to 70% in the next 32 min.The average recoveries of CPT in three levels were 100.02%,98.94% and 99.25%,The average recoveries of HCPT were 99.13%,99.66% and 98.97%.Using this assay,CPT and HCPT in leaves of Camptotheca acuminata Decne.in different seasons were determined.The results showed that the accumulation of CPT and HCPT presented evident relativity in the different growth stages.