PreS1抗原与HBeAg联合检测用于预测HBV-DNA的水平

摘要

目的:联合检测慢性乙型肝炎病毒(HBV)携带者的血清前S1(PreS1)抗原和乙肝e抗原(HBeAg)两项指标,判断其与HBV-DNA定量的相互关系.方法:采用双抗体夹心酶联免疫吸附分析(ELISA)法(两步法)检测PreS1抗原,双抗体夹心ELISA法(一步法)检测HBeAg,实时荧光定量聚合酶链反应(PCR)法检测HBV-DNA.结果:在335例慢性HBV携带者患者中,PreS1抗原阳性率为42.7％,HBeAg阳性率为36.4％,HBV-DNA阳性率为53.4％.在179例HBV-DNA阳性患者中,HBeAg阳性109例(60.9％)；156例HBV-DNA阴性患者中,HBeAg阴性143例(91.7％).在179例HBV-DNA阳性患者中,PreS1抗原阳性89例(49.7％)；在156例HBV-DNA阴性患者中,PreS1抗原阴性102例(65.4％).PreS1和HBeAg双阳性的感染者HBV-DNA阳性率为93.8％( 45/48)；在PreS1和HBeAg双阴性的感染者中,HBV-DNA阳性率仅为22.0％ (26/118).结论:HBeAg与HBV-DNA的相关性比PreS1抗原与HBV-DNA的相关性高.PreS1和HBeAg双阳性的感染者HBV的复制程度高,而双阴性的感染者复制程度较低.因此,PreS1抗原与HBeAg联合检测可用于预测HBV-DNA的水平.%Aim:To study the relationship of HBV-DNA with the HBV biomarkers PreSl and HBeAg in chronic HBV-carriers. Methods: PreSl and HBeAg were detected by sandwich ELISA. HBV-DNA was detected by fluorescence quantitative polymerase chain reaction assay. Results: Among 335 chronic HBV-carriers'serum samples,the positivety rate of preSl, HBeAg and HBV-DNA were 42.7% , 36.4% and 53. 4% , respectively. Meanwhile, the positivety rates of preSl and HBeAg in patients with serum HBV-DNA positive were 49.7% (89/179) and 60. 9% (109/179), respectively. The negative rates of preSl and HBeAg in patients with serum HBV-DNA negative were 65.4% (102/156) and 91. 7% (143/156), respectively. The positive rates of serum HBV-DNA in patients with PreSl and HBeAg both positive were 93. 8% (45/48) , while only 22.0% (26/118) in patients with PreSl and HBeAg both negative. Conclusion:There is a high correlation between HBeAg and HBV-DNA, indicating that HBeAg has a better concordance with HBV-DNA than PreSl. HBV carriers with PreSl and HBeAg both positively have high HBV-DNA copies. The co-detection of PreSl and HBeAg can predict the replication level of HBV.