藻油中DHA⁃EE的HPLC快速定量方法的建立

摘要

High performance liquid chromatography( HPLC) method was developed to quantify DHA?EE in marine microalgae oil quickly.The optimized quality parameters for DHA?EE analysis were as follows: chroma?tographic column, ZORBAX SB?C8( 4.6 mm×250 mm) ; mobile phase, methanol versus water ( 90∶10) ; the column temperature 30℃; the flow velocity 1.0 mL/min; UV wavelength 204 nm; the injection volume 10 μL. It presented a good linear relationship within range of 0.008 1?1.92 μg for DHA?EE, and the linear regression equation was y＝39.91 x+765.11( R2＝0.999 2). The average recovery rate was 102.42% ( n＝5, RSD＝2. 25%) with good precision( n＝10, RSD＝0.32%) , and the minimum quantify limit reached 8.05 ng.This opti?mized HPLC method could be used in the determination and analysis of algal oil and DHA?EE.%建立高效液相色谱法( high performance liquid chromatography, HPLC)快速测定藻油中二十二碳六烯酸乙酯(docosahexaenoic acid ethyl ester, DHA?EE)含量的方法.将藻油经乙酯化处理后,采用优化后DHA?EE分析参数:色谱柱ZORBAX SB?C8 (4.6 mm×250 mm),流动相V(甲醇)∶ V(水)＝90∶10 ,柱温为30 ℃,流速为1.0 mL/min,紫外检测波长为204 nm,进样量为10 μL.测定结果显示, DHA?EE在0.008 1～1.92 μg范围内呈良好线性关系,线性回归方程为: y ＝ 39.91 x+765.11(R2＝ 0.999 2) ,平均回收率为102.42%(n ＝ 5,RSD ＝ 2.25%) ,精密度(n ＝ 10,RSD ＝ 0.32%)较好, DHA?EE最低定量限达8.05 ng.表明,该方法可用于藻油及其制品中DHA?EE的检测分析.