松花粉的抗氧化作用

摘要

先对松花粉进行脱油、水提、冻干,获得水提物冻干品,再与缺陷型大肠杆菌菌液进行混合,稀释至终质量浓度为0.5,1.0,2.0 mg/mL,喂食模式生物秀丽隐杆线虫(线虫).通过测定线虫的寿命、产卵、吞咽及运动能力,确定松花粉水提物对线虫无明显毒性作用;通过10 mmol/L过氧化氢体外氧化压力诱导线虫,确定0.5,1.0,2.0 mg/mL松花粉水提物对线虫体外氧化压力有显著保护作用.%We obtained the lyophilized products of water extracts by removing oil,water extract, freeze drying pine pollen.The water extracts was diluted with Escherichia coli OP50 to a final mass concentration of 0.5,1.0,2.0 mg/mL to culture Caenorhabditis elegans .By measuring the lifespan, oviposition,swallowing,locomotion of C.elegans ,we determined that water extracts of pine pollen had no toxic effects on C.elegans . Through oxidative stress induced by 10 mmol/L hydrogen peroxide,we determined that 0.5,1.0,2.0 mg/mL water extracts of pine pollen had a significant protective effect on oxidative stress of C.elegans .