胶质瘤患者T淋巴细胞表面CTLA-4的表达及其临床意义

摘要

Objective:To investigate the correlation between the CTLA-4 expression in the peripheral blood CD4+ and CD8+T cells in the patients with glioma and glioma WHO classification and operation,and to clarify the clinical significances of CTLA-4.Methods:60 patients with glioma from our hospital were selected as glioma group, and 46 healthy volunteers were used as control group.Then the CTLA-4 expression levels in the peripheral blood CD4+ and CD8+T cells of the subj ects in glioma group and control group were detected by fluorescence-activated cell sorting (FACS)and the relationship between CTLA-4 and glioma WHO classification and surgery was analyzed. Results:The CTLA-4 expression levels in CD4+ and CD8+T cells in peripheral blood of the patients in glioma group were higher than those in control group (P<0.01).The CTLA-4 expression level in the patients with gradeⅣ glioma was the highest, the expression level of the patients with grade Ⅳ glioma was higher than that of the patients with grade Ⅲ glioma (P<0.01),and the expression level of grade Ⅲ the patients with was higher than that of the patients with grade Ⅱ (P<0.01).The expression level of CTLA 4 in CD4+ and CD8+T cells of the patients with glioma after operation was lower than before operation (P<0.01 ). Conclusion:The CTLA-4 expression levels in the peripheral blood CD4+ and CD8+T cells are increased with the increasing of malignancy degree of the patients with glioma,and it has a promotion role in the occurrence of development of glioma.%目的：探讨胶质瘤患者外周血中CD4＋和CD8＋T细胞表面CTLA-4表达与胶质瘤WHO分级及手术的关系，阐明CTLA-4的临床意义。方法：选择本院胶质瘤患者60例作为胶质瘤组，健康志愿者46名作为健康对照组。流式细胞术检测胶质瘤组和健康对照组研究对象外周血中 CD4＋和 CD8＋T细胞表面 CTLA-4的表达，分析其与胶质瘤 WHO分级及手术的关系。结果：胶质瘤组患者外周血 CD4＋和 CD8＋T细胞表面 CTLA-4的表达水平明显高于对照组（P＜0.01）；Ⅳ级胶质瘤患者 CD4＋和 CD8＋T 细胞表面 CTLA-4表达水平最高，Ⅳ级胶质瘤患者CD4＋和 CD8＋T 细胞表面 CTLA-4表达水平高于Ⅲ级胶质瘤（P＜0.01），Ⅲ级胶质瘤患者CD4＋和CD8＋T细胞表面CTLA-4表达水平高于Ⅱ级胶质瘤（P＜0.01）；术后胶质瘤患者CD4＋和CD8＋T细胞表面CTLA-4表达水平低于术前（P＜0.01）。结论：胶质瘤患者外周血CD4＋和CD8＋T细胞表面 CTLA-4的表达水平随着胶质瘤恶性程度的升高而升高，并在胶质瘤发生发展过程中发挥促进作用。