JC-1单染法检测CCCP对内皮细胞线粒体膜电位的影响

摘要

目的：分析碳酰氰基-对-氯苯腙（CCCP）对人脐静脉内皮细胞ECV304线粒体的影响。方法体外培养ECV304细胞，不同浓度CCCP处理细胞20 min后，装载荧光探针JC-1或Rh123，流式细胞术检测JC-1单体的发射绿色荧光。结果伴随线粒体毒性剂CCCP浓度增加，代表JC-1单体含量的绿色荧光强度增加。结论流式细胞术检测JC-1单体的绿色荧光可反应线粒体膜电位的变化。%Objective Analyze the influence of carbonyl cyano-to-chlorobenzene hydrazone ( CCCP ) on human um-bilical vein endothelial cells ECV-304 mitochondria .Methods ECV-304 cell was cultured in vitro and treated with different concentrations of CCCP for 20 minutes .After straining by fluorescent probes Rh 123 or JC-1 ,cells were meas-ured by flow cytometry .Results The intensity of green fluorescence emitted by JC-1 monomer increased with the in-creasing of CCCP concentration .Conclusion The changing of cell MMP could be well detected by flow cytometry staining by new fluorescent probe JC-1 .