Abcg4 was found highly expressed in the brain, eye and spleen by means of semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) , with higher expression in the first two organs. To further study the function of the gene, the effect of specifically designed small interference RNA (siRNA) on inhibiting this gene's expression was investigated in vivo. These siRNAs were synthesized and injected into the mouse lateral ventricle with different dosage. Brains were immediately removed at different time poinl after injection, and Ab-cg4 mRNA expression was measured by RT-PCR. The result shows that 6ug of the specific siRNA can inhibit the expression of Abcg4 significantly, with an inhibition rate up to 85% 48 hours after injection. The study concludes that the expression of Abcg4 can be inhibited by its specific siRNA in the mouse brain.%利用半定量RT-PCR的方法研究基因Abcg4在小鼠组织的表达情况,发现Abcg4基因在小鼠脑、眼睛和脾中表达,其中脑和眼睛中表达较高.为进一步研究Abcg4基因的功能,采用RNA干涉(RNAi)技术抑制脑中Abcg4基因的表达,通过体内实验了解其对Abcg4基因的沉默效率.设计合成针对Abcg4基因的小干扰RNA (siRNA),不同剂量siRNA注射小鼠侧脑室内,于小鼠注射后不同时相点,采用半定量RT-PCR法检测脑内Abcg4基因的表达量.结果表明,特定靶序列的siRNA注射剂量6μg,注射48 h时,对Abcg4基因的抑制效率可达85％.研究表明,脑注射siRNA可以显著抑制小鼠脑中Abcg4基因的表达.
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