一种离心式微流控生化分析芯片

摘要

In this paper, a low-cost, rapid, automated centrifugal microfluidic chip for biochemical analysis was presented. The chip integrates sample pre-processing and multi-biochemical items assay. Through multi-level microlfuidic channels and micro-siphon valves, the chip combines the pre-treatment of samples, sample transportation and the reaction processes between sample and biochemical reagent. The results are obtained by colorimetric detection. It is shown that the reproducibility of cuvette pathlength for chip is from 0.08% to 0.52%. The coefifcient of variation (CV) of sample, diluents metering and mixing for chip by the CV of blood glucose results is 1.4%. The CV of day-to-day and within-run precision for 15 biochemical items are less than 3.5%. Therefore, it is concluded that the centrifugal microlfuidic chip for biochemical analysis can satisfy the clinical testing requirements.%文章介绍了一种低成本、快速、自动化的离心式微流控生化分析芯片。该芯片通过整合样本前处理和多生化指标检测，采用了多级微流道与虹吸微阀相结合的方式把样本的前处理、样本输送、样本与生化试剂反应等过程集成于一体，并通过比色检测获得各个生化检测项目的结果。实验结果显示芯片比色孔光程的精密度的变异系数在0.08％～0.52％，通过血糖结果的精密度评估的芯片对样本和稀释液定量以及两者混合的精密度变异系数为1.4%，同时使用芯片测定15个生化项目的日间和批内精密度均小于3.5%，这表明该离心式微流控生化分析芯片符合临床检测要求。